Reconstitution Guide

Gather your materials

You will need: the lyophilised peptide vial, bacteriostatic water (BW), a sterile syringe (1 mL insulin syringe recommended), alcohol swabs, and appropriate PPE (gloves, eye protection).

Disinfect all surfaces

Wipe the rubber septum of both the peptide vial and the BW vial with a fresh alcohol swab. Allow to dry for 30 seconds. Work in a clean area; a laminar flow hood is ideal but not required for research use.

Draw bacteriostatic water

Draw the required volume of BW into your syringe (see the calculator below). For example: 1 mg peptide + 1 mL BW = 1 mg/mL concentration. For 5 mg vials, adding 2.5 mL BW gives a 2 mg/mL solution.

Inject slowly — against the glass

Insert the needle into the peptide vial. Angle so the stream of BW runs down the side of the glass rather than directly onto the lyophilised powder. Add the water slowly — never squirt forcefully. This prevents foaming and peptide damage.

Dissolve without agitation

Gently swirl (do not shake or vortex) until the powder is fully dissolved. If the solution is cloudy or particulate remains, allow it to sit at room temperature for 5–10 minutes before swirling again. Some peptides (especially GHK-Cu) may need slightly longer.

Inspect the solution

The reconstituted solution should be clear and colourless (GHK-Cu will be blue — this is normal). Do not use if visibly cloudy, has floating particles, or has an unusual colour after full dissolution.

Label and store

Label the vial with the date of reconstitution, compound name, and concentration. Store reconstituted peptides at 2–8°C (refrigerator). Most reconstituted peptides remain stable for 4–6 weeks when stored cold and protected from light.